Its content was about 9 . 8 % among total cell protein by gene genius bio imaging system . the fusion proteins were found largely in an insoluble inclusion bodies . the purified fusion proteins was obtained by his6 technique used to immunize rabbits to obtain polyclonal antiserum with titer of 1 * 105 经工ptg诱导，重组质粒在点co力‘ blzi中表达出了c端融合了6xhis的融合蛋白，过量表达的蛋白主要以不溶性蛋白形式存在，其表达量占菌体总蛋白的9 . 8 % 。
Different hosts " response suggested that tumv - sd1 could infect plants of 10 species in 3 families . tumv - sd1 formed pine - wheel inclusion bodies in plant cells . the coat protein of the tumv - sd1 contains 3 components whose estimated molecular weight are 45kd , 38kd and 14kd respectively 寄主反应特性表明， tumv - sd1 6能侵染3科10种植物， tumv - sd1在寄主细胞内形成风轮状内含体，外壳蛋白为3组分，分子量分别为45kd 、 38kd和14kd ；提纯的病毒粒体为长线条状。
After washing with reagent , adopt the newest purification technology source30rpc , sds - page and densitometric scan analysis , the result show that expression level is 90 % of total bacterial proteins . after renaturation , ifnr , hgfa , hgfb , hpk5 were purified by akta purifier chromatogram instrument , sepharose fast flow , ssphacrayl series gel , selecting optimize condition . finally establish a kind of high efficient purification model of recombinant proteins produced in escherichia coli as inclusion bodies , purification product purity > 98 % 结论:总之，通过对发酵罐中重组工程菌各种培养因素的研究，建立了一种高密度、高表达发酵工艺体系，为重组蛋白的后续纯化提供了大量、稳定的原料供应;通过对不同目的蛋白的色谱行为的系统研究，建立了一种高效稳定、快速简洁、易于放大的包涵体重组蛋白分离纯化体系。
Sectionii : to research and establish a kind of high efficient purification model of recombinant proteins produced in escherichia coli as inclusion bodies . to raise separate efficiency and biological active efficiency is especially important in the purification model of recombinant proteins . in accordance with these questions , we raise inclusion bodies purity before the purification as far as possible , and select the suitable chromatogram technology 研究并建立一种包涵体中高效分离纯化目的蛋白的优化模式重组蛋白分离纯化工艺中分离效率的提高、生物活性效率的提高及工艺的稳定性是尤其重要的，针对这些问题，我们在层析纯化前尽山西医科大学生物化学与分子生物学2003届硕士学位论文可能地提高包涵体纯度，并选择合适层析方法的同时合理的组合色谱分离单元。
The steady dead generation and time that was caused by the isolated virus was certain by chicken embryo which was inoculated on seven or nine days . the histopathological changs of the infectious stunting syndrom were studied by the way of ordinary paraffin section and he dying . the experimental result were as follows : the test proved that the changes of the chicken embryo were different in different stage . the chicken embryo dead in a week after it inoculated . the body was dropsy and hemorrhage . dead before it hatched out , the embyo body were dropsy , pale and slime . the liver was yellow and swolled , gallbladder ( vesica fellea ) was filled with bile . bursa and glandula thymus analosis . the kindey dropsy . bowel lamina were humble , dilatation . gas and yellow foam were filled the bowel . histopathological changes were that , in early stage , obvious changes of liver and kindey were dropsy , hemorrhage and necrosis . two types eosinophilic intranuclear inclusion bodies including large round and little granular were present in cells of the above organs . the obvious changes of bursa were dropsy , adverse folliiculated growth and little lymphocytes proliferating , 19 - 21 days chicken embryo , one or two big empty vacuoles were prensent in cells of liver and kindey . the number of the folliculi was growing , the vacuoles between cells were larger 胆囊充盈、其内充满稀薄的胆汁；法氏囊、胸腺萎缩，肠道扩张、肠壁菲薄、内充满气体及黄色泡沫状物；肾脏肿大。病理组织学变化方面，早期肝脏、肾脏、肠主要以出血、水肿和坏死为主，且肝细胞核及肾小管的上皮细胞核内均发现有核内包涵体，包涵体呈嗜酸性，为大型圆形包涵体或不规则的颗粒状；法氏囊则以水肿、滤泡发育不良、小型淋巴细胞数量增多为主。 19 21日龄鸡胚肝细胞、肾小管上皮细胞的胞浆内出现1 2各大的空泡，法氏囊滤泡数目增多细胞间有较大空隙。
This subject uses the genetic engineering technology and the newest modern biotechnology to study the genetic engineering lower reaches stage , in order to research and establish the optimum , high density fermentation technology pattern and high efficient isolation and purification model of recombinant proteins from inclusion bodies 本课题利用基因工程技术，重点从基因工程下游阶段着手研究，利用最新现代生物技术研究并建立经优化的高密度发酵工艺模式及高效分离纯化包涵体重组蛋白模式。一
The recombinant b . thuringiensis subsp . israelensis b - pmpx2 can produce a rhombus crystalline inclusion body during its sporulation sds - page analysis proved that cytlaa had overexpressed during the sporulation of the recombinant . it was found that b - pmpx2 strain remained stable toxicity , whatever during vegetative phase or sporulation phase , which was similar to the expression of mtxl gene in the strain of protease ( s ) - deficient b . sphaericus 同时，将来源于苏云金芽孢杆菌以色列亚种( b . thuringiensissubsp . israelensis , b . t . i )的cytlaa基因和p20伴侣蛋白基因克隆连接到质粒pmt9中，并转化到苏云金芽孢杆菌无晶体型中得到重组转化子b - pmpx2 ，电镜观察发现重组转化子b - pmpx2形成一菱形晶体。
The more favorable experiment conditions of preparing anatase nanometer tio2 powder are obtained from a lot of data . preparation technology of rutile nanometer tio2 powder is researched on the base of experiment of anatase nanometer tio2 powder . the influences of enclosure dose ' s quantity , preroasting temperatures phase - transition accelerant ' s quantity and calcining intensity and so on on the properties of inclusion body - zntio3 / ti ( oh ) 4 , granule size and properties of rutile nanometer tio2 powder are discussed 在锐钛型纳米tio _ 2粉体的制备基础之上，进一步研究了金红石型纳米tio _ 2粉体的制备工艺，探讨了包覆剂用量、预焙解温度、晶型促进剂量及焙烧温度和保温时间等因素对znco _ 3 / ti ( oh ) _ 4沉淀包覆体性能、金红石型纳米tio _ 2粉体产品的粒度和性能的影响。
The reults of sds - page and western blot dermonstrated that the insoulable component of the induced e . coli culture contained protein 3a . the results of the study indicated that protein 3a existed in the form of inclusion body . the content of the expressed protein in the induced bacteria protein was 29 . 2 % and 22 . 1 % respectively Sds - page和westernbolt结果证实，大肠杆菌菌体不可溶性蛋白中富含3a蛋白，且此融合蛋白的分子量符合预期设计，说明3a蛋白在表达产物中以包涵体的形式存在，所表达的蛋白含量分别占菌体蛋白的29 . 2和22 . 1 。
The purified enzyme had a specific activity of 68 . 6 u / mg protein . overproduction of pga was often limited by translocation and / or periplasmic processing steps , subsequently resulted in intracellular accumulation of various types of pga precursors and then formed inclusion bodies in the cytoplasm and / or periplasm 经deae - sepharosecl6b离子交换层析和butyl - sepharosecl4b疏水层析，即可得纯度提高20倍、比活为68 . 6u mg的青霉素g酰化酶，两步纯化的总得率达91 。
